Effect of Various Stabilizers on Titre of Lyophilized Live- Attenuated Peste Des Petits Ruminants (ppr) Vaccine M. Asim, A. Rashid and A. H. Chaudhary
نویسندگان
چکیده
Lyophilization stabilizes the biological materials by using two overlapping drying procedure i.e. primary drying by sublimation of the ice crystal from frozen material and secondary drying or desorption by evaporation of the free water adsorbed into the dried product. Three different stabilizers i.e. lactalbumin hydrolysate-sucrose, Weybridge medium and lactalbumin hydrolysate-manitol were used to lyophilize the Peste des petits ruminants (PPR) vaccine. Titre of live-attenuated PPR cell culture experimental vaccine was studied after lyophilization which revealed that PPR vaccine lyophilized with Weybridge medium was more stable and maintained the virus titre longer than rest of stabilizers used in the study.
منابع مشابه
Production of Homologous Live Attenuated Cell Culture Vaccine for the Control of Peste Des Petits Ruminants in Small Ruminants
Antibody response of a live-attenuated Peste des Petits Ruminants (PPR) cell culture vaccine was studied at Veterinary Research Institute, Lahore, Pakistan. For this purpose, one group of five sheep and 5 goats each was vaccinated subcutaneously with 1 ml reconstituted PPR vaccine and second group of five sheep and 5 goats was inoculated with 1 ml saline solution. Blood samples were collected b...
متن کاملCloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector
With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
متن کاملComparative Efficacy of Stabilizers on the Thermostability of Peste des Petits Ruminants Vaccine
Peste des petits ruminants (PPR) is an acute and highly contagious fatal disease of small ruminants mainly affecting sheep and goats, and camels. PPR is endemic disease in many developing and tropical countries including Pakistan, and vaccine is currently the only means of disease control. However, lack of established infrastructures and high temperature, maintaining cold chain for effective de...
متن کاملRaising of Rabbit Hyperimmune Serum for Laboratory Diagnosis of Peste Des Petits Ruminants (ppr) Virus
Agar gel immunodiffusion (AGID) test is a simple and rapid method for the diagnosis of Peste des petits ruminants (PPR) which requires the preparation of potent specific hyperimmune serum. This study deals with the raising of hyperimmune serum against PPR virus in rabbit. Four different inocula included PPR vaccine reconstituted in phosphate buffer saline, PPR antigen suspended in phosphate buf...
متن کاملPeste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review
Peste des petits ruminants (PPR) is caused by a Morbillivirus that belongs to the family Paramyxoviridae. PPR is an acute, highly contagious and fatal disease primarily affecting goats and sheep, whereas cattle undergo sub-clinical infection. With morbidity and mortality rates that can be as high as 90%, PPR is classified as an OIE (Office International des Epizooties)-listed disease. Consideri...
متن کامل